Fizykochemia miękkiej materii

Zespół badawczy nr 10

Fizykochemia miękkiej materii

Kierownik zespołu

prof. dr hab. Robert Hołyst

Tematyka badawcza

Analizujemy reakcje biochemiczne na poziomie pojedynczych molekuł stosując metody spektroskopowe ze zliczaniem pojedynczych fotonów. Badamy reakcje biochemiczne w probówce i w żywych komórkach. Oprócz tego rozwijamy nowe metody nierównowagowej fizyki statystycznej. Tworzymy również podłoża do SERSa.

Członkowie

  1. Prof. Robert Hołyst - Team Leader
  2. Patrycja Kuźma, Research Group Coordinator & Lab Manager
  3. Prof. Anna Maciołek
  4. Prof. Andrzej Poniewierski
  5. Dr. Karina Kwapiszewska
  6. Dr. Tomasz Kalwarczyk
  7. Dr. Grzegorz Bubak
  8. Dr. Tomasz Andryszewski
  9. Dr. Karol Makuch
  10. Dr. Paweł Żuk
  11. Dr. Jeffrey Everts
  12. Dr. Marta Pilz
  13. Dr. Patrycja Haniewicz
  14. Dr. Nima Farahmand Bafi
  15. Dr. Ewelina Kalwarczyk
  16. Paweł Albrycht, SERSitive
  17. Aneta Magiera, PhD Student
  18. Alicja Zgorzelska, PhD Student
  19. Karolina Kucharska, PhD Student
  20. Sakshi Sareen, PhD Student
  21. Adam Kowalski, PhD Student
  22. Katarzyna Ulewicz, PhD Student
  23. Jarosław Michalski, PhD Student
  24. Huma Jamil, PhD Student
  25. Uladzislau Sakalouski, PhD Student
  26. Antoni Lis, PhD Student
  27. Tomasz Piros, PhD Student
  28. Tadeusz Rymarz, PhD Student
  29. Michał Przerwa, PhD Student
  30. Jakub Wróbel, Student
  31. Daniel Czerniejewski, Student
  32. Paulina Obara, Student
  33. Tymon Obrębski, Student
  34. Jan Pyrzanowski, Student

Publikacje

2021

Richter, Ł., Księżarczyk, K., Paszkowska, K., Janczuk-Richter, M., Niedziółka-Jönsson, J., Gapiński, J., Łoś, M., Hołyst, R., & Paczesny, J.
Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research.
Scientific Reports, https://doi.org/10.1038/s41598-021-86571-x

Everts, J. C., & Ravnik, M.
Ionically Charged Topological Defects in Nematic Fluids.
Physical Review X, https://doi.org/10.1103/PhysRevX.11.011054

Agasty, A., Wisniewska, A., Kalwarczyk, T., Koynov, K., & Holyst, R.
Macroscopic Viscosity of Polymer Solutions from the Nanoscale Analysis.
ACS Appl. Polym. Mater., https://doi.org/10.1021/acsapm.1c00348

Kucharska, K., Pilz, M., Bielec, K., Kalwarczyk, T., Kuźma, P., & Hołyst, R.
Two Intercalation Mechanisms of Oxazole Yellow Dimer (YOYO-1) into DNA.
Molecules, https://doi.org/10.3390/molecules26123748

Kleczewska, N., Sikorski, P. J., Warminska, Z., Markiewicz, L., Kasprzyk, R., Baran, N., Kwapiszewska, K., Karpinska, A., Michalski, J., Holyst, R., Kowalska, J., & Jemielity, J.
Cellular delivery of dinucleotides by conjugation with small molecules: targeting translation initiation for anticancer applications.
Chemical Science, https://doi.org/10.1039/D1SC02143E

Al-Otaibi, J. S., Albrycht, P., Mary, Y. S., Mary, Y. S., & Księżopolska-Gocalska, M.

Concentration-dependent SERS profile of olanzapine on silver and silver-gold metallic substrates.

Chemical Papers, https://doi.org/10.1007/S11696-021-01783-9 

Kalwarczyk, T., Bielec, K., Burdzy, K., & Holyst, R.

Influence of molecular rebindings on the reaction rate of complex formation.

Physical Chemistry Chemical Physics, https://doi.org/10.1039/D1CP02820K

Al-Otaibi, J. S., Albrycht, P., Mary, Y. S., Mary, Y. S., & Księżopolska-Gocalska, M.

Concentration-dependent SERS profile of olanzapine on silver and silver-gold metallic substrates.

Chemical Papers, https://doi.org/10.1007/S11696-021-01783-9

Albrycht, P., Al-Otaibi, J. S., Mary, Y. S., Mary, Y. S., Trivedi, R., & Chakraborty, B.

Surface enhanced Raman scattering investigation of pioglitazone on silver and silver-gold metal substrates – Experimental analysis and theoretical modeling.

Journal of Molecular Structure, https://doi.org/10.1016/J.MOLSTRUC.2021.130992

Richter, Ł., Paszkowska, K., Cendrowska, U., Olgiati, F., Silva, P., Gasbarri, M., Guven, P., Paczesny, J., & Stellacci, F.
Broad-spectrum nanoparticles against bacteriophage infections.
Nanoscale, https://doi.org/10.1039/D1NR04936D

Aneta Karpińska, Marta Pilz, Joanna Buczkowska, J. Żuk, P., Karolina Kucharska, Gaweł Magiera, Karina Kwapiszewska, & Robert Hołyst
Quantitative analysis of biochemical processes in living cells at a single-molecule level: a case of olaparib–PARP1 (DNA repair protein) interactions.
Analyst, https://doi.org/10.1039/D1AN01769A

Stumpf, B. H., Nowakowski, P., Eggeling, C., Maciołek, A., & Smith, A.-S.
Protein induced lipid demixing in homogeneous membranes.
Physical Review Research, https://doi.org/10.1103/PhysRevResearch.3.L042013

Gizynski, K., Makuch, K., Paczesny, J., Zhang, Y., Maciołek, A., & Holyst, R.
Internal energy in compressible Poiseuille flow.
Physical Review E,  https://doi.org/10.1103/PhysRevE.104.055107

2022

Bielec, K., Kowalski, A., Bubak, G., Witkowska Nery, E., & Hołyst, R.
Ion Complexation Explains Orders of Magnitude Changes in the Equilibrium Constant of Biochemical Reactions in Buffers Crowded by Nonionic Compounds.
The Journal of Physical Chemistry Letters,  https://doi.org/10.1021/ACS.JPCLETT.1C03596

Araki, T., Gomez-Solano, J. R., & Maciołek, A. 
Relaxation to steady states of a binary liquid mixture around an optically heated colloid.
Physical Review E,  https://doi.org/10.1103/PhysRevE.105.014123

Khan, F., Jaoui, M., Rudziński, K., Kwapiszewska, K., Martinez-Romero, A., Gil-Casanova, D., Lewandowski, M., Kleindienst, T. E., Offenberg, J. H., Krug, J. D., Surratt, J. D., & Szmigielski, R.
Cytotoxicity and oxidative stress induced by atmospheric mono-nitrophenols in human lung cells.
Environmental Pollution,  https://doi.org/10.1016/J.ENVPOL.2022.119010



Żuk, P. J., Makuch, K., Hołyst, R., & Maciołek, A.  Transient dynamics in the outflow of energy from a system in a nonequilibrium stationary state.
Physical Review E,  https://doi.org/10.1103/PhysRevE.105.054133

Żuk, P. J. 
A brief introduction to contemporary electrokinetics.
Contemporary Physics, https://doi.org/10.1080/00107514.2022.2072565

Karpińska, A., Zgorzelska, A., Kwapiszewska, K., & Hołyst, R.
Entanglement of polymer chains in hypertonic medium enhances the delivery of DNA and other biomacromolecules into cells.
Journal of Colloid and Interface Science, https://doi.org/10.1016/J.JCIS.2022.07.040

Yasmeen, N., Karpinska, A., Kalecki, J., Kutner, W., Kwapiszewska, K., & Sharma, P. S.

Electrochemically Synthesized Polyacrylamide Gel and Core–Shell Nanoparticles for 3D Cell Culture Formation.

ACS Applied Materials & Interfaces, https://doi.org/10.1021/ACSAMI.2C04904

Pacocha, N., Zapotoczna, M., Makuch, K., Boguslawski, J., & Garstecki, P.
You will know by its tail: a method for quantification of heterogeneity of bacterial populations using single-cell MIC profiling.
Lab on a Chip, https://doi.org/10.1039/D2LC00234E

Kowalski, A., Bielec, K., Bubak, G., Żuk, P. J., Czajkowski, M., Sashuk, V., Huck, W. T. S., Antosiewicz, J. M., & Holyst, R.
Effective screening of Coulomb repulsions in water accelerates reactions of like-charged compounds by orders of magnitude.
Nature Communications, https://doi.org/10.1038/s41467-022-34182-z
The reaction speed of like-charged compounds in water is extremely slow due to Coulomb repulsions. Here, the authors boost kinetics up to 5 million times by screening these interactions and increasing the local concentration of reactants using positively charged micelles. They show the effect of two independent systems and present a theoretical explanation.

2023

Karpinska, A., Magiera, G., Kwapiszewska, K., & Hołyst, R.
Cellular Uptake of Bevacizumab in Cervical and Breast Cancer Cells Revealed by Single-Molecule Spectroscopy.
The Journal of Physical Chemistry Letters, https://doi.org/10.1021/ACS.JPCLETT.2C03590

van der Linden, M. N., Everts, J. C., van Roij, R., & van Blaaderen, A.
Realization of the Brazil-nut effect in charged colloids without external driving.
Proceedings of the National Academy of Sciences, https://doi.org/10.1073/PNAS.2213044120
A colloidal Brazil-nut effect was realised using charged colloids. Unlike its' granular counterpart no external shaking is needed to observe this phenomenon.

Ochirbat, E., Zbonikowski, R., Sulicka, A., Bończak, B., Bonarowska, M., Łoś, M., Malinowska, E., Hołyst, R., & Paczesny, J.
Heteroaggregation of virions and microplastics reduces the number of active bacteriophages in aqueous environments.
Journal of Environmental Quality,  https://doi.org/10.1002/JEQ2.20459

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