Offer no. 28/14
New conducting bis(bithiophene) polymer molecularly imprinted with neopterin, the method of its preparation and application of this polymer as a recognition unit of a chemosensor for selective detection and determination of neopterin
The invention involves a conducting molecularly imprinted polymer (MIP) composed of 2,2'-bithiophene-5-boronic acid and 2-(cytosin-1-yl)ethyl p-bis(2,2'-bithien-5-yl)methylbenzolate as the functional monomers, and 2,4,5,2',4',5'-hexa(thiophene-2-yl)-3,3'-bithiophene as the cross-linking monomer, and molecular cavities imprinted with neopterin molecules as well as the method of its preparation by electropolymerization under potentiodynamic conditions. Moreover, the invention includes the use of the molecularly imprinted polymer as a recognition unit of a chemosensor for detection and selective determination of neopterin, especially in biological samples including blood and urine. The chemosensor potentiometrically transduces a chemical signal of neopterin recognition to the useful analytical signal manifested by the change of an open circuit potential (OPC).
Piyush S. Sharma, Agnieszka Wojnarowicz, Marta Sosnowska, Francis D'Souza, Włodzimierz Kutner
- Advantages / Innovative aspects:
- A conducting molecularly imprinted polymer (MIP) for selective determination of neopterin, a cancer biomarker, was devised and prepared.
- Combination of the potentiometric signal transduction with the recognition using the tailor designed MIP film resulted in devising an easy to prepare and robust in use chemosensor.
- The procedure of neopterin determination developed with the use of the chemosensor is superior in comparison to those conventional engaging HPLC and radioimmunoassays because these assays are time consuming and expensive.
- The devised chemosensor successfully discriminates neopterin from its structural analogues.
- The chemosensor successfully determines neopterin in the human serum samples, thus confirming its suitability in medical/clinical analysis.
Cancer biomarker, serum neopterin, open circuit potential, molecularly imprinted polymer, MIP, chemosensor, chemical sensor, clinical diagnosis
The MIP-neopterin chemical sensor devised and fabricated for selective and sensitive detection and determination of neopterin, a cancer biomarker, especially in body fluids, such as blood serum and urine, can be used for early diagnosis of different cancers including the colon, ovarian, endometrial, cervical, renal, and breast cancer.
The method of neopterin determination developed with the use of the proposed chemosensor can be alternative to conventional methods, such as HPLC and radioimmunoassays. The reported HPLC procedures include a preliminary acid deproteinization step, which may lead to the decrease of the amount of neopterin in a sample. Unfortunately, these assays are time consuming and expensive. In contrast, the developed method is fast, easy to operate, and inexpensive.
Clinical research, Diagnostics, Physiology, Oncology, Measuring sensors
- State of the progress:
stage of research
- Intellectual property rights:
Patent in Poland