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Lipid nanoparticles (LNPs) are crucial for delivering nucleic acids into cells, powering mRNA vaccines and gene editing. While generally considered low-toxic (>24h), we observed significant intracellular nanostructure remodeling and diffusion hindrance within 4 hours of LNP exposure. Using Fluorescence Correlation Spectroscopy (FCS), we found these disturbances far exceed those from other stressors. We hypothesize that LNP lipids interact with cell membranes, causing this remodeling, potentially impacting organelle health and cellular stress. This project will use a soft matter approach and high-throughput biochemical assays, to analyze LNP-cell interactions and their biological consequences. We aim to understand the physicochemical impact of LNPs on cell membranes (1-100nm scale) and connect these findings to standard toxicity measures. This research will reveal potential adverse LNP effects, crucial for both molecular biology and clinical applications. 

The responsibilities of  the stipend holder will include:

1. Performing experiments (cytotoxic biochemical assays for microplate reader/flow cytometry)
2. Data analysis (calculating cytotoxicity).  

We offer a scholarship position in the Institute of Physical Chemistry PAS with a monthly stipend of 1000 PLN  financed from the OPUS 27 No. 2024/53/B/ST4/01213 entitled “Effect of lipid nanoparticles on the nanostructure of cell cytoplasm”.   The stipend is planned for 24 months with possible extensions.

The selected student will gain valuable new knowledge and learn techniques that will strengthen their practical skill set. This project combines biophysics, nanotechnology, cell biology, and physical chemistry, offering a broad learning opportunity for individuals from diverse backgrounds. Participation in the research will also provide hands-on experience and the chance to complete an exciting thesis project. We intend to publish the results in prestigious, high-impact journals.

1. At the starting date of the work within the project, the applicant should be a student of full-time first or second-cycle degree programmes or uniform Master’s studies at any university in Poland,
2. Knowledge of fluorescence and biochemistry,
3. Ability to communicate in English,
4. Strong motivation to work and solving scientific problems, creativity, and analytical thinking.

The recruitment committee shall evaluate the candidates’ research achievements, their research-related achievements and competencies as regards specific tasks in the research project, expressed as a point score:

• the candidate’s research achievements, including publications in prestigious academic press /journals (50% of the final score)
• research achievements, scholarships, awards and research experience gained in Poland or abroad, research workshops and training courses, participation in research projects (20% of the final score)
• the candidate’s competence to carry out specific tasks in the research project (30% of the final score)