Equipment database
- Name:
- Confocal microscope
- Producer:
- Nikon
- Model:
- C1
- Research group:
- Team 10. Soft condensed matter
- Description:
-
The Nikon C1 Confocal Microscope is a device for measuring fluorescence correlation using spectroscopy. It is equipped with a PicoHarp 300 fluorescence correlation spectroscopy module from PicoQuant and two detectors based on avalanche photovoltaics. The microscope is built into a chamber that allows for the control of measurement temperature and atmosphere (gases: nitrogen, CO2). It is possible to mount an environmental control chamber to measure as a function of temperature.
- Contact:
dr Tomasz Kalwarczyk
Research
- Type of measurement: Confocal imaging
- Test method: Laser beam scanning
Details
- Name of equipment: Nikon model: C1
- Type of measurement: Confocal imaging
- Test method: Laser beam scanning
- Opis:
Measurement is based on simple confocal imaging of fluorescent preparations. It is possible to build a three- dimensional model of the test specimen. No analytical capabilities of the built image.
- User performing the measurement: dr Tomasz Kalwarczyk
- Type of measurement: Measurement of diffusion coefficient of fluorescent probes in the tested environment
- Test method: Fluorescence correlation spectroscopy
Details
- Name of equipment: Nikon model: C1
- Type of measurement: Measurement of diffusion coefficient of fluorescent probes in the tested environment
- Test method: Fluorescence correlation spectroscopy
- Opis:
The study consists of analyzing the fluctuation of the concentration of the fluorescent analyte in confocal volume. On this basis, using the appropriate algorithms, implemented in the software included with the apparatus, the mean time of fluorophore residence time in the test volume is determined. Then, on this basis, the coefficient of diffusion of the test substance is determined using the Einstein- Smoluchowski equation.
- Accuracy of measurement: not less than 10%
- User performing the measurement: dr Tomasz Kalwarczyk
- Type of measurement: Fluorescence Lifetime Imaging (FLIM)
- Test method: Scanning with laser light pulses
Details
- Name of equipment: Nikon model: C1
- Type of measurement: Fluorescence Lifetime Imaging (FLIM)
- Test method: Scanning with laser light pulses
- User performing the measurement: dr Tomasz Kalwarczyk