Institute of Physical Chemistry Polish Academy of Sciences, Warsaw, Poland TEAM 10: Soft Condensed Matter Group
Łukasiewicz Research Network - PORT Polish Center for Technology Development
Project title, type and project number
Horizon for Excellence in messenger RNA applications in immunoOncology, Virtual Research Institute (VIB) no. WIB-1/2020-O11, 4/2022
Prof. Andrzej Dziembowski – Leader of WIB_HERO project, Prof. Robert Hołyst – Team Leader of IChF Group in WIB_HERO project
Most current experimental methods for real-time measurements in single cells provide only qualitative and semiquantitative insights into the molecular mechanisms of interest. More detailed data can be obtained in high-throughput assays (biochemical assays with cell extracts) or from studies on fixed cells (various labelling procedures). This technological gap prevents the understanding of the kinetics of subcellular processes in particular. This gap can be filled by a technique that uses Fluorescence Correlation Spectroscopy (FCS), which allows a determination of the number and the diffusion time of single molecules in a sample. This well-known technique has found limited application in living cells due to the difficulty in interpreting data regarding "diffusion time" in particular. Robert Hołyst's group has conducted research for over 10 years to develop an empirical model linking the size of molecules and the hydrodynamic resistance they encounter in complex fluids, including the cytoplasm of cells and the interior of the cell nucleus. This model and the resulting equation allow direct calculation of diffusing molecules inside the cell. This allows us to determine the number of moving molecules and their average size and their number.
The goal of the project will be the application of advanced fluorescence techniques to answer fundamental questions about interactions of exogenous mRNA in the cellular interior. To this end, the original, proprietary method for real-time measurements of molecular interaction parameters in living cells will be used. This method is based on fluorescence correlation spectroscopy (FCS), supported by quantitative fluorescence microscopy and fluorescence lifetime imaging (FLIM).
We are looking for an experimentalist with solid theoretical background in chemistry to cope with the physicochemical problems in physical chemistry. We guarantee access to top-notch instrumentation and well-equipped laboratories.
Number of vacancies
Key responsibilities include
1. Sample preparation
3. Data analysis
Role in the project:
Position 1 - Biophysical use of single molecule fluorescence methods to analyze the stability and translation of mRNA in living cells.
Position 2 - Biophysical use of the fluorescence method of an alternative molecule for analysis of endosomes and translation of mRNA in living systems.
Position in the project
Postdoctoral Researcher position
Full-time employment contract
We offer a monthly salary up to PLN 15000 (depending on experience and qualifications). This amount shall be subject to obligatory deductions for health and social insurance, advance income tax payments, and other public dues of the employer and the emplo
Position starts on
Maximum planned period of contract/stipend agreement
Career development prospects at IPC PAS
Career development prospects at IChF:
Chemistry, Physical Chemistry
Scientific profile of a candidate
Recognised Researcher (R2)
Requirements for the candidate – the candidate:
Key assessment criteria
The commission evaluates applications on a point scale (max 100 points).
The Commission will take into account the following criteria:
Top candidates will be invited for an online or in-person interview. Good command of English is required. We reserve the right to contact and reply to only selected candidates.
Only candidates with a minimum of 70 points would be considered for the position. A position will be offered to the person who obtains the highest number of points. The Recruitment Committee may not recommend any candidate for the position if no candidate would get at least 70 points.
1. Professional curriculum vitae, including:
2. a cover letter not exceeding 3,500 characters (1 A4 page) containing concise information on scientific / professional interests, achievements to date, possible participation in research projects and own research interests,
3. scans of University degree diploma and PhD Diploma (chemistry or related discipline),
4. the candidate's declaration that he/she has become acquainted with the General Rules Governing Competitions for Research Posts at IChF in Warsaw.
Candidates will be selected in an open competition, complying with the principles underlying the “European Charter for Researchers” and the “Code of Conduct for the Recruitment of Researchers” as a part of IChF “HR Excellence in Research” strategy.
The position will also be awarded also in accordance with the granting agency regulations: Standardy rekrutacji do Zespołów Badawczych w ramach Wirtualnego Instytutu Badawczego oraz przeprowadzanie ewaluacji ich stosowania, the IChF employment policy and the General Rules Governing Competitions for Research Posts at the IPC PAS in Warsaw.
How to Apply: Send application directly to firstname.lastname@example.org.
IMPORTANT! email subject: “Recruitment no. 4/2022”
The Selection Committee evaluates applications and interviews candidates on a point scale. The position will be awarded to the person who obtains the highest number of points.
At the employment starting date, the applicant should be enrolled to a doctoral school.
The position will be awarded to the person who obtains the highest number of points.
If the top candidate resigns, we reserve the right to choose the next candidate from the ranking list.
The results of the competition are made public.
The competition may be extended until a suitable candidate who fulfils all requirements is recruited.
The announcement will be published on the following websites:
Candidate have the right to file a complaint about any action in the recruitment process. The appeal shall be lodged with the Director of the Institute within 7 days from the date of receipt of the appropriate information. The decision of the Director of the Institute is final.
More information about the VIB:
More information about the IPC PAS:
More information about the Team: https://softmatter.ichf.edu.pl/
Deadline for the settlement of the competition
Archival announcement. We would like to thank all candidates for their submitted applications.