Institute of Physical Chemistry PAS
National Science Center (NSC)
Project title, type and project number
Microfluidic methods for quantitative antibiotic susceptibility assays with single cell resolution; MAESTRO 10 Project No. 2018/30/A/ST4/00036
prof. Piotr Garstecki
The objective of the project is to develop an analytical platform to encapsulate bacterial cells into droplets using microfluidics, incubate them and detect growth without optical labeling. In the envisioned protocol, the sample culture of cells will be serially diluted and each dilution split and mixed with multiple concentrations of antibiotic to screen inoculum density and antibiotic concentration with digital droplet assays. The assays at high and low inoculum density will allow us to count the fraction of populated droplets that produced dense colonies of bacteria. These counts will allow us to measure the single-cell Minimum Inhibitory Concentration (scMIC) level and the inoculum effect (IE) while the low-density screens will uncover the phenotypic heteroresistance, p(scMIC). The method will offer a wealth of information for research on the response of cells and bacterial colonies to antibiotics, and hopefully will guide more effective treatments regimens.
Key responsibilities include
Position starts on
Maximum planned period of contract/stipend agreement
The position is for the period of 32 months (with the possibility to extend to 36 months if the project is prolonged by NCN).
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Key assessment criteria
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