WP4 - Biospectroscopy - platforms for molecular diagnostics

Leader - Prof. Jacek Waluk
vice-leader - Dr. Agnieszka Michota-Kami
Institute of Physical Chemistry of the Polish Academy of Sciences
Task 4.1 Transfer of knowledge and experience through twinning and networking (visits)
Establishing and developing two-way secondments of research staff between the IPC PAS and four
knowledgeable and experienced partnering organisations to improve IPC PAS research potential in the following areas of vibrational spectroscopy:
. SERS detection and identification of neurotransmitters
. Immunoassays utilizing SERS
. DNA and gene probes based on SERS
. SERS studies inside living human cells
. SERS-active substrates for medical applications
. Fundamental aspects of enhancement of Raman spectra
Twinning agreements will be signed between IPC PAS and the following academic research institutions (indicated below head of group or someone from his/her group ):
1. National Biophotonics and Imaging Platform, National Centre for Sensors Research, Dublin City University,Dublin, Ireland. Professor Tia Keyes (nanoscale interfacial platforms for surface enhanced bio-Ramanspectroscopy and enhanced luminescence, photochemistry, supramolecular interfacial chemistry
2. Institute of Physical and Theoretical Chemistry, Eberhard-Karls University of TŘbingen, Germany, Prof. Alfred J. Meixner (Nano-Optics, Optical Single-Molecule Spectroscopy, advanced laser spectroscopy at the nanometerscale)
3. Ultrafast Photochemistry Group (UPG), Departement of physical chemistry of the University of Geneva, Switzerland. Prof. Eric Vauthey (ultrafast spectroscopy, dynamics of photoinduced electron transfer and photopolymerisation reactions)
4. Department of Chemistry, Katholieke Universiteit Leuven, Belgium, Prof. Johan Hofkens (single molecule microscopy and their application in material and bio-sciences).

Secondments of research staff members:
1 week 1 month 2 months 3 months 4 months
DCU 3(2012,2013,2014) 1(2014)
IPTC 2(2012,2013) 2(2012,2013,
UPG 3(2012,201, 2014) 2(2012,2013)
DCUL 2(2013,2014) 1 (2014)
1 week 1 month 2 months 3 months 4 months
DCU 2(2012,201) 1(2014)
IPTC 3(2012,2013,2014) 1(2012)
UPG 3(2012,2013,2014) 1(2013)
DCUL 1 (2014) 1(2013),
Total secondment staff: 14,75 P/M
Task leader: Dr. Agnieszka Kami

Task 4.2 Recruitment of one experienced researcher
To develop the scientific capabilities and human potential of IPC-PAS in the vibrational spectroscopy area we are planning to recruit one experienced researcher for 18 months in the field of Raman spectroscopy. The candidate for experienced researcher position is expected to be an active researcher and have track- record of significant research achievements appropriate to the field and at least matching one or more of following benchmarks:
. Publications in major international peer-reviewed scientific journals,
. Satisfactory number of citation,
. Invited presentations in well established international conferences,
. International practice in the sense of scholarship outside the country of applicant,
. Capability in knowledge transfer
Leader Task: Prof. Jacek Waluk

Task 4.3 Starting independent research group by young researchers IPC PAS
Creation of an independent research group formed by young researchers, supported by an experienced advisor, into increasing potential of ICP-PAS for development of nanomaterials science platform in ICP-PAS in the field of:
. Developing and optimization of new efficient SERS platform for medical applications,
. SERS gene probe for medical diagnosis,
. Detection of DNA mutations using Raman spectroscopy,
. SERS nanoparticle tags for in vivo imaging.
Task leader: Prof. Jacek Waluk

Task 4.4. Facilitation of participation of the IPC PAS researchers in international scientific conferences
At least three international conferences, workshops and symposia will be targeted, among them:
. 25th European Conference on Biomaterials, Madryt, 2013.
. 23rd International Conference on Raman Spectroscopy, Bangalore, India, 2012.
- This is the highest profile international conference on Raman spectroscopy for the duration of the project.
. Paris International Conference on Nanoscience and Nanotechnology, Paris, France, 2012.
Facilitated participation at the event will be possible under condition of having at least one talk lecture/ research report will be presented.
Task leader: Dr. Agnieszka Kami˝ska

Task 4.5. Organization of the Conference: International Conference on Advanced Infrared and Raman Spectroscopy in the framework of Polish Photoscience Seminars"
A high -profile International Conference (in 2012) will bring together researchers, applications scientists and instrumentation developers from universities, research institutes and industry to provide a forum to present and discuss recent developments in all fields of vibrational spectroscopy. Topic to be presented and discussed (one or more from the list): IR and Raman microscopy, biochemistry, structural biology and medicine, high-resolutionspectroscopy, near-field techniques, non-linear Raman spectroscopies, spectroscopic imaging, surface spectroscopies and new materials, time-resolved and coherent methods, two-dimensional techniques, new results and novel techniques in spectroscopy, photochemistry, photophysics, photobiology, photomedicine and photodiagnostics, materials science, and environmental protectionvibrational optical activity. A key aim is to encourage students and young people at the early stage of their careers in the area of vibrational spectroscopy.
Advances Infrared and Raman Spectroscopy, 16-18 November 2012; (report)
Task leader: Prof. Jacek Waluk

Task 4.6. Workshop: Applications of Raman Spectroscopy
The one-day workshop (2014) will give an introduction to Raman spectroscopy. This will include the theoretical basics, practical training in the use of spectrometers, introduction to data reduction and interpretation, and an overview of applications in various fields of materials science, biomedical, pharmaceutical, medical (diagnostic, in vivo), mineralogy, geology and other fields of sciences. A number of current-day applications of Raman Spectroscopy will be presented in more detail through invited talks by experts in the field. The workshop aims at putting participants in the position to apply Raman spectroscopy in their own research. For the laboratory training, participants will be encouraged to bring own samples for analysis.
Expected date: January - March 2014
Expected cost: 12,600€
Expected number of participant: 30-100
Task leader: Dr. Agnieszka Kaminska

Total estimated costs for WP 4 - 300,107€
Report No 1 - December 2011

Report No 2 - March 2012
Raport No 3 - June 2012
Raport No 4 - September 2012
Raport No 5 - December 2012

Report No 6 - March 2013

Report No 7 - June 2013

Report No 8 - September 2013

Report No 9 - December 2013

Report No 10 - March 2014

Report No 11 - June 2014

Report No 12 - September 2014